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Updated: Feb 19, 2026
Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells
Published on: September 15, 2014
Lithium Chloride can Induce Differentiation of Human Immortalized RenVm Cells into Dopaminergic Neurons
Mitra Soleimani1, Nazem Ghasemi1
1Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Background:
Stem cell-based therapy is a novel strategy for the treatment of neurodegenerative diseases. The transplantation of fully differentiated cells instead of stem cells in order to decrease serious adverse complications of stem cell therapy is a new idea. In this study, the effect of lithium chloride on dopaminergic differentiation of human immortalized RenVm cells was investigated in order to access a population of fully differentiated cells for transplantation in Parkinson disease.
Methods:
The immortalized RenVm cells were induced to dopaminergic differentiation using a neurobasal medium supplemented with N2 and different concentrations (1, 3, 6 mM) of Lithium Chloride (LiCl) for 4, 8 and 12 days. The efficiency of dopaminergic differentiation was evaluated using immunocytochemistry and western blot techniques for tyrosine hydroxylase and β-catenin marker expression.
Results:
Our results indicated that LiCl can promote dopaminergic differentiation of RenVm cells in a dose-dependent manner.
Conclusion:
It can be concluded that LiCl is able to facilitate dopaminergic differentiation of cultured cells by affecting Wnt-frizzled signaling pathway.
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