[Ganoderma triterpenoids from aqueous extract of Ganoderma lucidum]

Area of Science:

• Natural Product Chemistry
• Phytochemistry
• Medicinal Plants

Background:

• Ganoderma lucidum, a traditional medicinal mushroom, is known for its diverse bioactive compounds.
• Previous research has identified numerous triterpenoids and polysaccharides from Ganoderma lucidum.
• The chemical constituents of its water decoction, a common traditional preparation, warrant further comprehensive investigation.

Purpose of the Study:

• To isolate and characterize novel and known chemical constituents from the water extract of Ganoderma lucidum.
• To contribute to the complete chemical investigation of Ganoderma lucidum water decoction.
• To enrich the knowledge base of Ganoderma lucidum's phytochemical profile.

Main Methods:

• Water extract of Ganoderma lucidum subjected to sequential column chromatography (silica gel, ODS, Sephadex LH-20).
• High-Performance Liquid Chromatography (HPLC) used for final purification.
• Extensive spectroscopic data (NMR, MS) and physicochemical properties utilized for compound elucidation.

Main Results:

• A new triterpenoid, 2β-acetoxy-3β,25-dihydroxy-7,11,15-trioxo-lanost-8-en-26-oic acid (1), was isolated and characterized.
• Eighteen known analogues, including various ganoderic and lucidenic acids, were identified and confirmed.
• The study successfully identified 19 compounds from the water extract, including the novel compound.

Conclusions:

• The isolation of a new triterpenoid and 18 known compounds expands the known chemical diversity of Ganoderma lucidum.
• This research provides a comprehensive chemical profile of the traditional water decoction of Ganoderma lucidum.
• The findings contribute significantly to the understanding of Ganoderma lucidum's phytochemistry and potential therapeutic applications.