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Detection of True IgE-expressing Mouse B Lineage Cells
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Mouse Eosinophils: Identification, Isolation, and Functional Analysis.

Hadar Reichman1, Perri Rozenberg1, Ariel Munitz1

  • 1Department of Clinical Microbiology and Immunology, The Sackler School of Medicine, Tel-Aviv University, Ramat Aviv, Israel.

Current Protocols in Immunology
|November 2, 2017
PubMed
Summary
This summary is machine-generated.

This study details methods for identifying and isolating eosinophils, crucial immune cells involved in inflammation and immunity. These techniques enable better functional assessment of eosinophils in research settings.

Keywords:
chemotaxiseosinophilsflow cytometryinflammation

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Eosinophils are bone marrow-derived immune cells regulated by interleukin-5 (IL-5).
  • They play key roles in mucosal tissues during allergic inflammation, parasitic infections, and cancer.
  • Functional studies of eosinophils are challenging due to their terminal differentiation and rapid in vitro death.

Purpose of the Study:

  • To establish simple and effective methods for characterizing and obtaining eosinophils for functional studies.
  • To provide detailed protocols for identifying tissue eosinophils and isolating them from bone marrow.

Main Methods:

  • Flow cytometry for identifying tissue eosinophils.
  • Detailed protocols for isolating eosinophils from bone marrow.
  • Methods for differentiating eosinophils for functional assessment.

Main Results:

  • Established methodology for identifying eosinophils in tissues via flow cytometry.
  • Developed detailed procedures for isolating eosinophils from bone marrow.
  • Provided techniques for preparing eosinophils for functional studies.

Conclusions:

  • Simple methods for eosinophil identification, isolation, and functional assessment have been successfully described.
  • These techniques are vital for advancing research on eosinophil functions in various physiological and pathological conditions.