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MicroRNA-150 promotes cell proliferation, migration, and invasion of cervical cancer through targeting PDCD4

Zhan Zhang1, Jinming Wang2, Jing Li2

  • 1The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, PR China; Shangqiu Medical College, Shangqiu, Henan 476000, PR China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|November 2, 2017
PubMed

Insights

MicroRNA-150 (miR-150) is elevated in cervical cancer and drives cancer growth by targeting Programmed Cell Death Protein 4 (PDCD4). This suggests miR-150 could be a therapeutic target for cervical cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • MicroRNA-150 (miR-150) is upregulated in various cancers, including cervical cancer.
  • The precise role of miR-150 in regulating cervical cancer cell proliferation, migration, and invasion remains to be fully elucidated.

Purpose of the Study:

  • To investigate the mechanism underlying miR-150's role in cervical cancer progression.
  • To determine the relationship between miR-150 and Programmed Cell Death Protein 4 (PDCD4) in cervical cancer.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) and Western blot to assess miR-150 and PDCD4 expression.
  • Transwell and MTT assays to evaluate cell migration, invasion, and proliferation.
  • Luciferase reporter assay to confirm PDCD4 as a direct target of miR-150.

Main Results:

  • miR-150 expression was significantly increased in cervical cancer tissues and serum compared to normal controls.
  • PDCD4 expression was markedly decreased in cervical cancer tissues.
  • Upregulation of miR-150 promoted cervical cancer cell proliferation, migration, and invasion, mediated by the targeting of PDCD4.

Conclusions:

  • miR-150 promotes cervical cancer progression by downregulating PDCD4.
  • miR-150 may serve as a potential diagnostic and therapeutic biomarker for cervical cancer.
Keywords:
Cervical cancerInvasionMiR-150MigrationPDCD4Proliferation

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