Related Experiment Video
Updated: Feb 19, 2026

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
Published on: February 11, 2019
pStab: prediction of stable mutants, unfolding curves, stability maps and protein electrostatic frustration
Soundhararajan Gopi1, Devanshu Devanshu1, Praveen Krishna1
1Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras (IITM), Chennai 600036, India.
Summary:
We present a web-server for rapid prediction of changes in protein stabilities over a range of temperatures and experimental conditions upon single- or multiple-point substitutions of charged residues. Potential mutants are identified by a charge-shuffling procedure while the stability changes (i.e. an unfolding curve) are predicted employing an ensemble-based statistical-mechanical model. We expect this server to be a simple yet detailed tool for engineering stabilities, identifying electrostatically frustrated residues, generating local stability maps and in constructing fitness landscapes.
Availability And Implementation:
The web-server is freely available at http://pbl.biotech.iitm.ac.in/pStab and supports recent versions of all major browsers.
Contact:
athi@iitm.ac.in.
Supplementary Information:
Supplementary data are available at Bioinformatics online.
Related Concept Videos
Protein Folding
Protein Folding
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Molecular Chaperones and Protein Folding
The...
Molecular Chaperones and Protein Folding
Conserved Binding Sites
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
Bacterial Protein Maturation

