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Published on: September 14, 2013
Spore Preparation Protocol for Enrichment of Clostridia from Murine Intestine
Eric M Velazquez1, Fabian Rivera-Chávez1, Andreas J Bäumler1
1Department of Medical Microbiology and Immunology, School of Medicine, University of California at Davis, One Shields Avenue, Davis, CA 95616, USA.
Abstract:
In recent years, many spore-forming commensal Clostridia found in the gut have been discovered to promote host physiology, immune development, and protection against infections. We provide a detailed protocol for rapid enrichment of spore-forming bacteria from murine intestine. Briefly, contents from the intestinal cecum are collected aerobically, diluted and finally treated with chloroform to enrich for Clostridia spores.
