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Updated: Feb 19, 2026

Seven Steps to Stellate Cells

Seven Steps to Stellate Cells

Published on: May 10, 2011

Human hepatic stellate cell isolation and characterization.

Linshan Shang¹, Mojgan Hosseini², Xiao Liu³

¹Department of Medicine, University of California, San Diego, La Jolla, USA.

Journal of Gastroenterology

|November 3, 2017

Summary

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Mesothelin/Mucin 16 Signaling in Activated Portal Fibroblasts Drives the Development of Cholestatic Fibrosis and Hepatocellular Carcinoma in Aged Female Multidrug Resistance Protein 2 Knockout Mice.

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This review details human hepatic stellate cells (HSCs), crucial in liver fibrosis. It highlights challenges in translating rodent findings to human HSCs and discusses human HSC isolation, markers, and cell lines.

Area of Science:

Hepatology
Cell Biology
Translational Medicine

Background:

Hepatic stellate cells (HSCs) are key players in liver function and fibrosis.
Current understanding relies heavily on rodent models, posing translational challenges.
Species-related differences hinder the direct application of rodent findings to human liver diseases.

Purpose of the Study:

To review current methods for isolating human HSCs.
To identify reliable markers for human HSCs.
To discuss established human HSC cell lines for research.

Main Methods:

Literature review of human HSC isolation techniques.
Analysis of established human HSC markers.
Compilation of data on available human HSC cell lines.

Keywords:

Human HSC isolation Human HSC markers Human hepatic stellate cell lines Human hepatic stellate cells

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Last Updated: Feb 19, 2026

Seven Steps to Stellate Cells

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Published on: May 10, 2011

Isolation of Lung Retinoid-Containing Cells by Cell Sorting

Isolation of Lung Retinoid-Containing Cells by Cell Sorting

Published on: April 11, 2025

Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells

Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells

Published on: March 30, 2016

Main Results:

Various methods exist for human HSC isolation, each with limitations.
Specific markers are crucial for accurate human HSC identification.
Several human HSC cell lines are available, offering valuable research models.

Conclusions:

Accurate human HSC isolation and identification are critical for liver fibrosis research.
Established human HSC cell lines provide essential tools for studying human liver diseases.
Bridging the gap between rodent and human studies is vital for therapeutic advancements.