Immunophenotyping: Application to Safety Assessment
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Updated: Aug 14, 2026

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
Published on: March 25, 2014
11 Comparative Biology and Safety Sciences, Amgen, Inc., South San Francisco, California, USA.
Abstract:
A continuing education course entitled "What You Always Wanted to Know About Immunotoxicology in Pharmaceutical Development…But Were Afraid to Ask" was offered at the Society of Toxicologic Pathology (STP) 36th annual symposium in Montreal. This article summarizes some key points made during the presentation dedicated to immunophenotyping. It describes how clusters of differentiation (CDs) are well-defined antigens used to characterize cell subsets, and how lymphocyte subsets in humans and different rodent and nonrodent species can be defined by detection of various combinations of CDs. It provides an overview of immunophenotyping study design considerations and applications to safety assessment.
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