[Effects of sevoflurane on the migration of vascular endothelial cells]

To evaluate the effects of sevoflurane on cell migration and expression of VE-cadherin mRNA in human umbilical vein endothelial cells(HUVECs).

HUVECs were allocated to 4 groups randomly (each group contained 4 culture dishes with a diameter of 10 cm), and were exposed to 2% sevoflurane (refer to 1.6 MAC) respectively for 0 h (control group which exposed to the same environment as the other 3 groups but without sevoflurane), 0.5 h, 1 h, 2 h. After sevoflurane exposure, HUVECs were subjected to scratch assay to observe the migration distance; RT-PCR was used to detect the expression of vascular endothelial-cadherin mRNA(VE-cadherin mRNA). Statistical analysis of the data was conducted using SPSS 13.0 software package.

Scratch assay results suggested that the migration ability of HUVECs exposed to 2% sevoflurane for 2 h were significantly inhibited during 12 h after scratch compared with the control group(P<0.01), and RT-PCR results suggested that HUVECs exposed to 2% sevoflurane for 2 h expressed greater VE-cadherin mRNA than the control group(P<0.05).

Sevoflurane exposure for 2 h can inhibit HUVECs migration and the mechanism may be up-regulating VE-cadherin expression.