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Updated: Feb 19, 2026

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
Published on: March 20, 2018
[Interaction of phenylethanoid glycosides and calf thymus DNA]
Huan Yuan1, Tao Wang1, Chen-Chen Zhu2
1School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
Abstract:
In physiological condition, the interaction of acteoside and forsythoside B with calf thymus DNA using neutral red (NR) as a fluorescence probe were investigated by fluorescence, UV-visible spectrophotometry, viscosity, DNA melting techniques, and molecular docking. It is observed that acteoside and forsythoside B can react with DNA. The major mode of recognition between drug and DNA is groove binding by hydrogen bonds, and the interaction of acteoside with DNA is stronger than that of forsythoside B.
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