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[Study on effect of Jiangtang decoction on AGEs-RAGE and oxidative stress in KK-Ay mice]
Jin-Ni Hong1, Wei-Wei Li1, Hong Fu1
1Integrated Laboratory of Traditional Chinese Medicine and Western Medicine, Peking University, First Hospital, Beijing 100034, China.
Abstract:
To elucidate the efficacy of Jiangtang decoction(JTD) on AGEs-RAGE and oxidative stress in type 2 diabetic model KK-Ay mice. Fifty KK-Ay mice were randomly divided into 5 groups as follows： model group, metformin group, low-dose, medium-dose and high-dose of JTD group, with 10 C57BL/6J as normal group. All groups are orally administrated with equal distilled water, 250 mg•kg⁻¹ metformin hydrochloride, 2, 4,8 g•kg⁻¹ JTD, equal distilled water respectively, once per day for 12 weeks. Alanine aminotransferase(ALT), creatinine(CREA), urea nitrogen(BUN),advanced glycation end products(AGEs) and receptor of glycation end products(RAGE) in blood or urine were measured during the experiments. Furthermore, on the day of the sacrifice, kidney was collected, and electronic microscopy and immunohistochemistry were performed to evaluate the protective renal effect of JTD. In addition, the levels of AGEs, RAGE, Cata-lase(CAT) and superoxide dismutase(SOD) were assessed by Western blot, Real-time PCR or ELISA to analyze the efficacy of JTD. This study demonstrated that JTD might protect kidney of KK-Ay by down-regulating the expression of AGEs, RAGE and oxidative stress.
Insights
Jiangtang decoction (JTD) may protect kidneys in type 2 diabetes by reducing advanced glycation end products (AGEs) and receptor for AGEs (RAGE), thereby alleviating oxidative stress.
Area of Science:
- Pharmacology
- Nephrology
- Metabolic Disorders
Background:
- Type 2 diabetes is associated with increased risks of kidney damage.
- Advanced glycation end products (AGEs) and oxidative stress play critical roles in diabetic nephropathy.
- The therapeutic potential of traditional Chinese medicine, like Jiangtang decoction (JTD), warrants investigation.
Purpose of the Study:
- To investigate the efficacy of Jiangtang decoction (JTD) in mitigating AGEs-RAGE pathways and oxidative stress in a type 2 diabetic mouse model.
- To evaluate the protective effects of JTD on kidney function and structure in KK-Ay mice.
Main Methods:
- KK-Ay mice, a model for type 2 diabetes, were treated with varying doses of JTD or metformin for 12 weeks.
- Biochemical markers (ALT, CREA, BUN, AGEs, RAGE) were measured in blood and urine.
- Kidney tissues were analyzed using electron microscopy and immunohistochemistry.
- Levels of AGEs, RAGE, catalase (CAT), and superoxide dismutase (SOD) were assessed via Western blot, Real-time PCR, and ELISA.
Main Results:
- JTD treatment led to a reduction in AGEs and RAGE levels in KK-Ay mice.
- JTD demonstrated a protective effect on kidney structure and function.
- The decoction modulated oxidative stress markers, including CAT and SOD, indicating an antioxidative effect.
Conclusions:
- Jiangtang decoction (JTD) exhibits potential therapeutic benefits for diabetic nephropathy.
- JTD may exert its protective effects by down-regulating AGEs-RAGE signaling and reducing oxidative stress in the kidneys.
