Simultaneous Detection of Glutathione and Hydrogen Polysulfides from Different Emission Channels

Glutathione (GSH) and hydrogen polysulfides (H₂S_n) play crucial roles in many physiological processes. To unravel the complicated interrelationship and cellular cross-talk between GSH and H₂S_n, the development of single-molecule fluorescent probes that can selectively sense GSH and H₂S_n simultaneously from different emission channels is highly desirable. In this report, we have developed the first dual-detection fluorescent probe, ACC-SePh, which responded to GSH with green fluorescence emission, whereas it reacted with H₂S_n and emitted blue fluorescence. The probe exhibited excellent selectivity and sensitivity toward GSH and H₂S_n over other common reactive sulfur species, such as Cys, Hcy and H₂S. Importantly, we also demonstrated that ACC-SePh can be used for dual-channel imaging of endogenous GSH and H₂S_n in living RAW264.7 cells.