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Characterization of phyto-nanoparticles from Ficus krishnae for their antibacterial and anticancer activities
Amarvani P Kanjikar1, Aruna L Hugar1, Ramesh L Londonkar1
1a Department of Biotechnology, Biopharmaceutical and Nanobiotechnology Laboratory , Gulbarga University , Kalburagi , India.
Background:
Ovarian cancer is deadliest of fifth leading cause of death in women worldwide. This is due to advanced-stage disease rate associated with the development of chemoresistance. Hence, the current study emphasizes the process of synthesis of silver nanoparticles (AgNPs) from green chemistry method. Ficus krishnae is a perennial plant, native to India, used in folklore medicine to treat various diseases.
Objective:
For the development of reliable, ecofriendly, less expensive process for the synthesis of AgNPs against bacterial and ovarian cancer.
Methodology:
The synthesis of silver nanoparticles from stem bark of Ficus krishnae was carried out. The synthesized nanoparticles are subjected by UV-Vis spectrophotometer, scanning electron microscopy (SEM), X-ray diffraction (XRD) analysis and FTIR analysis. The antibacterial efficacy also determined by disc diffusion method, MIC, CFU and growth curve. In vitro cytotoxicity effect of aqueous extract and AgFK nanoparticle in ovarian cancer cell line by MTT assay was performed.
Results:
The formation of AgNPs was confirmed by UV-VIS spectroscopic absorbance shown that peak at 435 nm. XRD photograph has indicated the face-centered cubic structure of the synthesized AgNPs. SEM study demonstrated that the size from 160 to 260 nm with interparticle distance, whereas shape is spherical. The particle size were ranging from 15 to 28 nm determined by XRD pattern. The antibacterial and cytotoxicity activity of this nanoparticle has showed a potential activity when compared with standards.
Conclusion:
The present study confirms that the biosynthesized AgNPs from Ficus krishnae stem bark extract have a great affiance as antibacterial and anticancer agent.
Insights
Green synthesis of silver nanoparticles (AgNPs) from Ficus krishnae shows potential antibacterial and anticancer properties. This eco-friendly method offers a promising alternative for treating ovarian cancer and infections.
Area of Science:
- Nanotechnology
- Green Chemistry
- Pharmacology
Background:
- Ovarian cancer is a leading cause of death in women, often linked to chemoresistance.
- Developing effective and eco-friendly treatments is crucial.
Purpose of the Study:
- To synthesize silver nanoparticles (AgNPs) using a green chemistry approach from Ficus krishnae.
- To evaluate the antibacterial and anticancer efficacy of these AgNPs.
Main Methods:
- Biosynthesis of AgNPs using Ficus krishnae stem bark extract.
- Characterization using UV-Vis, SEM, XRD, and FTIR.
- Assessment of antibacterial activity via disc diffusion, MIC, and growth curves.
- Evaluation of in vitro cytotoxicity against ovarian cancer cell lines using MTT assay.
Main Results:
- UV-Vis confirmed AgNP formation at 435 nm.
- XRD indicated a face-centered cubic structure, with particle sizes ranging from 15-28 nm.
- SEM revealed spherical nanoparticles (160-260 nm).
- Synthesized AgNPs demonstrated significant antibacterial and cytotoxic activity.
Conclusions:
- Biosynthesized AgNPs from Ficus krishnae exhibit potent antibacterial and anticancer properties.
- This study highlights the potential of Ficus krishnae-derived AgNPs as therapeutic agents.
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