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Related Concept Videos

Rab Proteins01:14

Rab Proteins

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Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
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Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

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Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
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Rab Cascades01:25

Rab Cascades

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Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
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Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.4K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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Protein Modifications in the RER01:26

Protein Modifications in the RER

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Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
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Translational Regulation01:29

Translational Regulation

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Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
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Related Experiment Video

Updated: Feb 19, 2026

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
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Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

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Post-translational modifications: How to modulate Rab7 functions.

Graziana Modica1, Stephane Lefrancois1,2

  • 1Centre INRS-Institut Armand-Frappier, Institut National de la Recherche Scientifique, Laval, Québec, Canada H7V 1B7.

Small Gtpases
|November 4, 2017
PubMed
Summary
This summary is machine-generated.

Rab7 GTPase controls late endosome trafficking. Post-translational modifications like phosphorylation and palmitoylation fine-tune Rab7 interactions, regulating key cellular processes such as autophagy.

Keywords:
Rab7palmitoylationphosphorylationpost-translational modificationsubiquitination

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Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins
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In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells
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In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells

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Related Experiment Videos

Last Updated: Feb 19, 2026

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Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins
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In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells
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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Rab7 GTPase is a key regulator of membrane trafficking in late endosomes.
  • It controls the movement of molecules from endosomes to the Golgi and the fusion of endosomes with lysosomes.
  • Rab7 also plays a role in autophagosome-to-lysosome fusion during autophagy.

Purpose of the Study:

  • To review the current understanding of how Rab7 function is regulated.
  • To explore the role of post-translational modifications in modulating Rab7 effector interactions.
  • To discuss how these modifications orchestrate membrane trafficking pathways.

Main Methods:

  • Literature review of recent research on Rab7.
  • Analysis of studies investigating post-translational modifications of Rab7.
  • Discussion of experimental evidence linking modifications to effector binding and trafficking.

Main Results:

  • Rab7's function is modulated by a combination of post-translational modifications.
  • Phosphorylation, palmitoylation, and ubiquitination can selectively alter Rab7's binding to downstream effectors.
  • These modifications allow for precise control over distinct trafficking pathways.

Conclusions:

  • Post-translational modifications are crucial for fine-tuning Rab7 activity.
  • Understanding these modifications provides insight into the regulation of late endosome trafficking and autophagy.
  • Rab7's adaptability through modifications allows it to coordinate diverse cellular functions.