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Arthrobacter paludis sp. nov., isolated from a marsh
Qi Zhang1, Mihee Oh1, Jong-Hwa Kim1
1Department of Microbiology, Chung-Ang University College of Medicine, Seoul 06974, Republic of Korea.
International Journal of Systematic and Evolutionary Microbiology
|November 4, 2017
Summary
A new bacterium, Arthrobacter paludis, was discovered in Korean hydric soil. This strictly aerobic, Gram-positive bacterium represents a novel species within the Arthrobacter genus.
Area of Science:
- Microbiology
- Bacterial Taxonomy
- Environmental Microbiology
Background:
- Soil ecosystems harbor diverse microbial communities.
- The genus Arthrobacter is known for its metabolic versatility and prevalence in various environments.
- Characterizing novel bacterial isolates is crucial for understanding microbial diversity and ecological roles.
Purpose of the Study:
- To isolate and characterize a novel bacterium from a hydric soil sample.
- To determine the taxonomic position of the novel isolate using polyphasic characterization.
- To propose a new species designation for the isolate based on genotypic and phenotypic data.
Main Methods:
- Isolation and cultivation of bacteria from hydric soil.
- Phylogenetic analysis based on 16S rRNA gene sequences.
- Chemotaxonomic analyses including polar lipid profiling, fatty acid analysis, and cell wall composition.
- DNA-DNA hybridization and DNA G+C content determination.
Main Results:
- A novel Gram-positive, aerobic bacterium, strain CAU 9143T, was isolated.
- Phylogenetic analysis placed strain CAU 9143T within the genus Arthrobacter, closely related to Arthrobacter ginkgonis.
- Chemotaxonomic data (menaquinone, polar lipids, fatty acids) and DNA-DNA relatedness (22.3%) supported its distinction from known species.
- Optimal growth conditions were identified as 30°C, pH 7.0, and 1% NaCl.
Conclusions:
- Strain CAU 9143T represents a novel species of the genus Arthrobacter.
- The proposed name for the novel species is Arthrobacter paludis.
- This discovery expands the known diversity within the Arthrobacter genus and highlights the microbial potential of hydric soil environments.
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