Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cross-reactivity00:42

Cross-reactivity

33.2K
Overview
33.2K
Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

1.6K
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...
1.6K
Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

84.6K
Overview
84.6K
Antigen Presenting Cells01:22

Antigen Presenting Cells

3.6K
The immune system is a complex network of cells and molecules that protects the body from foreign invaders. T cells, a type of white blood cell, play a crucial role in this process. They recognize and attack foreign substances, such as pathogens, that enter the body.
T cells require the help of antigen-presenting cells (APCs), which process foreign antigens into smaller fragments that can be recognized by T cells. These APCs are highly specialized cells that efficiently internalize antigens...
3.6K
Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

1.7K
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
1.7K
Vaccinations01:51

Vaccinations

52.0K
Overview
52.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A comprehensive safety evaluation of approved 2'-fucosyllactose for human nutritional applications in China.

Frontiers in toxicology·2026
Same author

miR-4485-5p regulates the progression of periodontitis by targeting CDK9.

Odontology·2026
Same author

Nighttime Organic Nitrates Drive New Particle Formation and Aerosol Growth in Urban Beijing.

Environmental science & technology·2026
Same author

Application of tubeless mini-percutaneous nephrolithotomy under local anesthesia and lateral position in ambulatory surgery: a pilot feasibility study.

Scientific reports·2026
Same author

Rational design and modification strategies for pitch-derived carbon anodes for use in sodium-ion batteries.

Chemical communications (Cambridge, England)·2026
Same author

Heterogeneous impacts of work-family conflict on workforce mental health: evidence from the differential effects of work-to-family and family-to-work conflict.

Frontiers in public health·2026

Related Experiment Video

Updated: Feb 19, 2026

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein
08:04

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein

Published on: December 20, 2017

7.4K

Protective cellular immunity generated by cross-presenting recombinant overlapping peptide proteins.

Lili Cai1, Jianbo Zhang2, Renying Zhu1

  • 1Oxford Vacmedix (Changzhou) Company Ltd, Changzhou, Jiangsu, China.

Oncotarget
|November 5, 2017
PubMed
Summary

Recombinant overlapping peptides (ROPs) effectively stimulate CD8+ and CD4+ T cells for therapeutic vaccines. This novel technology shows promise in activating immune responses against cancer and infectious diseases.

Keywords:
antigen processingcross-presentationoverlapping peptidepeptide immunizationvaccines

More Related Videos

Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation
12:48

Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation

Published on: August 21, 2017

8.6K
HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL
07:18

HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL

Published on: April 11, 2011

15.9K

Related Experiment Videos

Last Updated: Feb 19, 2026

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein
08:04

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein

Published on: December 20, 2017

7.4K
Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation
12:48

Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation

Published on: August 21, 2017

8.6K
HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL
07:18

HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL

Published on: April 11, 2011

15.9K

Area of Science:

  • Immunology
  • Vaccinology
  • Molecular Biology

Background:

  • Dendritic cells (DCs) prime naive CD8+ and CD4+ T cells via MHC class I and II antigen presentation.
  • Effective T cell priming is crucial for robust immune responses.
  • Existing methods may have limitations in stimulating both T cell types simultaneously.

Purpose of the Study:

  • To develop and evaluate a novel recombinant overlapping peptide (ROP) technology for stimulating both CD8+ and CD4+ T cell responses.
  • To assess the potential of ROPs as a platform for therapeutic vaccines and diagnostics.

Main Methods:

  • Designed synthetic genes encoding ROPs from model antigens (ovalbumin, CFP10-ESAT6, HPV E7) and a tumor-associated antigen (survivin).
  • Utilized cathepsin S cleavage sites within ROPs for antigen processing in DCs.
  • Tested ROPs in vitro with human DCs and PBMCs, and in vivo in mouse immunization models.

Main Results:

  • ROP-derived epitopes were cross-presented and detected by CD8+ T cell receptor-like antibodies.
  • Human DCs pulsed with ROP-survivin activated CD8+ T cells.
  • PBMCs from co-infected patients recognized ROP-CFP10-ESAT6.
  • ROP immunization in mice generated specific cellular immunity and protected against tumor challenge.

Conclusions:

  • Recombinant overlapping peptides (ROPs) represent a viable strategy for simultaneous stimulation of CD8+ and CD4+ T cells.
  • ROP technology shows potential as a versatile platform for developing therapeutic vaccines and diagnostic tools.
  • Further development of ROPs could advance treatments for infectious diseases and cancer immunotherapy.