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Related Experiment Video

Updated: Feb 19, 2026

Author Spotlight: Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging

Author Spotlight: Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging

Published on: September 22, 2023

Tracking maize pollen development by the Leaf Collar Method.

Kevin Begcy¹, Thomas Dresselhaus²

¹Cell Biology and Plant Biochemistry, Biochemie-Zentrum Regensburg, University of Regensburg, 93053, Regensburg, Germany. kevin.begcy@ur.de.

Plant Reproduction

|November 5, 2017

Summary

A new Leaf Collar Method allows non-destructive identification of maize pollen development stages. This reproducible technique aids research into critical cellular events during pollen formation in this vital grass species.

Keywords:

Maize Meiosis Mitosis Nondestructive method Pollen development Sperm cell

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Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

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Last Updated: Feb 19, 2026

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Published on: September 22, 2023

Kinematic Analysis of Cell Division and Expansion: Quantifying the Cellular Basis of Growth and Sampling Developmental Zones in Zea mays Leaves

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Published on: December 2, 2016

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

Area of Science:

Plant Biology
Developmental Biology
Genetics

Background:

Pollen development involves crucial cellular events like meiosis and epigenetic modifications.
Studying these events in maize is challenging due to pollen's location within the plant.
A non-destructive method is needed to isolate specific pollen developmental stages.

Purpose of the Study:

To describe an easy, reproducible, non-destructive method for identifying maize pollen development stages.
To enable detailed molecular and physiological analyses of pollen development.

Main Methods:

The Leaf Collar Method was developed and applied to the maize inbred line B73.
Anther and tassel size, along with pollen stage percentages, were correlated with vegetative stages.
Stage-specific gene identification confirmed method reproducibility.

Main Results:

The Leaf Collar Method successfully identified and characterized distinct stages of maize pollen development.
The duration of each stage, from pollen mother cells to mature pollen, was determined.
Correlations between pollen stages and easily recognizable vegetative stages were established.

Conclusions:

The Leaf Collar Method provides a highly reproducible, non-destructive approach to study maize pollen development.
This method facilitates future research on transcriptomics, metabolomics, and epigenetics during pollen development.
It supports investigations under normal and stressful conditions in maize, a key agricultural species.