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Decellularization and Recellularization of Whole Livers
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Liver Bioengineering Using Decellularized Whole-Liver Scaffolds.

Iris Pla-Palacín1, Pilar Sainz-Arnal1,2, Sara Morini1,3

  • 1Instituto de Investigación Sanitaria de Aragón (IIS Aragón), Zaragoza, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|November 5, 2017
PubMed
Summary
This summary is machine-generated.

Whole-organ bioengineering offers a solution for organ transplantation shortages. This chapter details pig liver bioengineering techniques, including decellularization and stem cell recellularization, serving as a model for human applications.

Keywords:
DecellularizationLiver scaffoldOrgan bioengineeringPerfusion bioreactor systemRecellularization

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Area of Science:

  • Regenerative Medicine
  • Bioengineering
  • Transplantation Biology

Background:

  • Organ transplantation faces critical shortages, driving the need for alternative solutions.
  • Whole-organ bioengineering is emerging as a promising alternative to address organ scarcity.
  • Pig organs, particularly the liver, are valuable models for human applications due to physiological similarities.

Purpose of the Study:

  • To describe key techniques for whole-organ bioengineering of the pig liver.
  • To establish the pig liver as a model for future human liver bioengineering.
  • To outline methods for scaffold preparation and cell seeding for liver regeneration.

Main Methods:

  • Porcine whole-liver decellularization to create a natural scaffold.
  • Isolation of endothelial and mesenchymal stem cells from porcine sources.
  • Generation of hepatoblasts from porcine embryonic stem cells.
  • Scaffold recellularization using a bioreactor perfusion system for cell distribution and viability.

Main Results:

  • Successful decellularization of the pig liver, preserving extracellular matrix structure.
  • Isolation and characterization of relevant porcine stem cell populations.
  • Demonstration of hepatoblast differentiation potential.
  • Establishment of a bioreactor system for recellularizing the decellularized liver scaffold.

Conclusions:

  • Pig liver bioengineering techniques are advancing, offering a viable preclinical model.
  • The described methods provide a foundation for developing bioengineered organs for transplantation.
  • Further research in this area holds significant potential for overcoming organ donor limitations.