Related Experiment Video
Updated: Feb 19, 2026

A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants
Published on: January 1, 2016
Improved liquid chromatographic method for quality control of spiramycin using superficially porous particles
Qi Lin1, Getu Kahsay2, Tom de Waal1
1KU Leuven, University of Leuven, Department of Pharmaceutical and Pharmacological Sciences, Pharmaceutical Analysis, Herestraat 49, O&N2, PB 923, B-3000 Leuven, Belgium.
Abstract:
This article describes the development and validation of a liquid chromatographic method for spiramycin using a column with superficially porous particles. Gradient elution was applied and the mobile phase consisted of phosphate buffer (0.2M; pH 8.3) - H2O - acetonitrile in a ratio 10:60:30 (v/v/v) for mobile phase A and 10:30:60 (v/v/v) for mobile phase B. UV detection was performed at 232nm. Compared to previous methods, the analysis time was about two times faster and impurities were better separated. Furthermore, impurities which were present above 0.25% were characterized using liquid chromatography coupled with mass spectrometry (LC/MS).
Related Concept Videos
Supercritical Fluid Chromatography
SFC utilizes a supercritical fluid mobile phase,...
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
High-Performance Liquid Chromatography: Instrumentation

