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Touch DNA sampling with SceneSafe Fast™ minitapes.

Britta Stoop¹, Priscille Merciani Defaux², Silvia Utz¹

¹Institute of Forensic Medicine, Forensic Molecular Biology Dpt., University of Bern, Sulgenauweg 40, 3007 Bern, Switzerland.

Legal Medicine (Tokyo, Japan)

|November 6, 2017

Summary

Forensic tape-lifting for touch DNA collection is effective, especially SceneSafe Fast™ minitapes, outperforming swabs. Phenol-chloroform extraction yielded the best DNA results from tape-lifted samples.

Keywords:

Clothing DNA extraction Sampling Tape Trace DNA

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Area of Science:

Forensic Science
Molecular Biology
Genetics

Background:

Optimal trace DNA analysis relies on effective collection and extraction methods.
Tape-lifting is a common, non-destructive DNA collection technique, but its efficiency compared to swabbing and optimal extraction methods require further investigation.
Limited data exists on DNA extraction from forensic lifting tapes.

Purpose of the Study:

To compare the performance of SceneSafe Fast™ minitapes against wet cotton swabs for touch DNA collection from cotton fabric.
To evaluate the efficiency of phenol-chloroform extraction versus two automated magnetic bead-based methods (iPrep Forensic Kit and PrepFiler Express BTA™ Kit) for DNA extracted from tape lifts.

Main Methods:

Touch DNA was collected from cotton fabric using SceneSafe Fast™ minitapes and wet cotton swabs.
Standardized saliva samples were applied to minitapes for extraction method comparison.
DNA extraction was performed using phenol-chloroform and two magnetic bead-based kits: iPrep Forensic Kit and PrepFiler Express BTA™ Kit.

Main Results:

SceneSafe Fast™ minitapes demonstrated superior performance in collecting touch DNA compared to wet cotton swabs.
Phenol-chloroform extraction was the most efficient method for recovering DNA from saliva samples on SceneSafe Fast™ minitapes.
Single-pass tape application was sufficient for significantly enhanced collection compared to swabbing.

Conclusions:

SceneSafe Fast™ minitapes are an effective tool for touch DNA collection, outperforming traditional swabbing methods.
Conventional phenol-chloroform extraction remains a highly efficient method for forensic DNA recovery from tape lifts.
Further research into optimizing DNA extraction from tape lifts is warranted to enhance forensic trace DNA analysis.