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Related Experiment Videos

Platelet-derived growth factor: purification and partial characterization.

C H Heldin, B Westermark, A Wasteson

    Proceedings of the National Academy of Sciences of the United States of America
    |August 1, 1979
    PubMed
    Summary
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    Researchers isolated a cationic protein from human platelets that stimulates DNA synthesis in cultured human cells. This growth factor, composed of two polypeptides, shows potent activity comparable to human serum.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Molecular Biology

    Background:

    • Human platelets contain growth factors crucial for cellular processes.
    • Stimulating DNA synthesis is key to cell proliferation and tissue repair.

    Purpose of the Study:

    • To isolate and characterize a cationic protein from human platelets that stimulates DNA synthesis.
    • To determine the protein's composition and biological activity.

    Main Methods:

    • Ion exchange chromatography
    • Hydrophobic chromatography
    • Gel chromatography
    • Sodium dodecyl sulfate-gel electrophoresis

    Main Results:

    • A cationic protein stimulating DNA synthesis was purified from human platelets.

    Related Experiment Videos

  • The native protein (30,000 daltons) consists of two polypeptides (13-14 kDa and 16-17 kDa) linked by reducible bonds.
  • The purified protein exhibited potent stimulatory activity on human glial cells at approximately 4 ng/ml.

    • Conclusions:

    • A novel cationic growth factor from human platelets stimulates DNA synthesis in human cells.
    • The purified protein is a potent mitogen for human glial cells.