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Regulation of Expression at Multiple Steps

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...

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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

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Related Experiment Video

Updated: Feb 19, 2026

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

Reading m⁶A in the Transcriptome: m⁶A-Binding Proteins.

Deepak P Patil¹, Brian F Pickering¹, Samie R Jaffrey²

¹Department of Pharmacology, Weill Medical College, Cornell University, New York, NY 10065, USA; These authors contributed equally.

Trends in Cell Biology

|November 7, 2017

Summary

This summary is machine-generated.

N6-Methyladenosine (m6A) is a key RNA modification. YTH domain proteins bind m6A, influencing RNA fate and impacting the epitranscriptome through diverse cellular functions.

Keywords:

N(6)-methyl adenosine RNA metabolism YTH proteins m(6)A modification splicing translational regulation

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Area of Science:

Molecular Biology
Epigenetics
RNA Biology

Background:

N6-Methyladenosine (m6A) is the most abundant epitranscriptomic mark on eukaryotic RNA.
m6A modifications are dynamically regulated and play crucial roles in gene expression.
Proteins with YTH domains are key readers of the m6A mark.

Purpose of the Study:

To elucidate the mechanisms by which YTH domain proteins recognize and bind m6A.
To investigate how YTH domain proteins influence the fate of m6A-modified RNA.
To review the diverse functions of YTH domain proteins in mammalian cells.

Main Methods:

Analysis of protein-RNA interactions.
Investigating molecular mechanisms of m6A recognition.
Review of existing literature on YTH domain protein functions.

Main Results:

YTH domain proteins specifically bind to m6A-modified RNA.
m6A binding by YTH proteins affects RNA stability, splicing, and translation.
Diverse and sometimes opposing roles of YTH proteins in cellular processes were observed.

Conclusions:

YTH domain proteins are critical mediators of m6A RNA functions.
Understanding YTH protein mechanisms is key to deciphering the epitranscriptome.
Further research is needed to resolve the complex roles of these proteins.