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Related Experiment Video

Updated: Feb 19, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
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Fluorescent Probes and Labels for Cellular Imaging.

Christoph J Fahrni

    Chimia
    |November 7, 2017
    PubMed
    Summary

    Researchers developed new fluorescent probes for detecting zinc and copper in cells. These advanced indicators offer high sensitivity and selectivity for visualizing trace metals at the subcellular level.

    Area of Science:

    • Analytical Chemistry
    • Cell Biology
    • Biochemistry

    Background:

    • Metal-responsive fluorescent indicators are crucial for visualizing trace metals within cells.
    • Organic fluorophores enable the design of selective and sensitive metal ion probes.
    • Subcellular resolution is key for understanding metal ion function and dysregulation.

    Purpose of the Study:

    • To review challenges in designing metal-responsive fluorescent probes.
    • To present novel reagents developed for detecting zinc and copper in mammalian cells.
    • To highlight advancements in cellular metal ion imaging.

    Main Methods:

    • Rational design of organic fluorophores with tailored photophysical properties.
    • Development of selective and sensitive reagents for specific metal ions (zinc and copper).
    Keywords:
    excited state proton transferimagingphotoinduced electron transfertrace metalsx-ray fluorescence microscopy

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  • Application of probes for visualizing metal ions at the subcellular level in mammalian cells.

    • Main Results:

    • Successful design of fluorescent indicators for zinc and copper detection.
    • Demonstration of high selectivity and sensitivity of the developed reagents.
    • Visualization of trace metal distribution and dynamics within mammalian cells.

    Conclusions:

    • Advanced fluorescent probes are essential for studying cellular metal homeostasis.
    • The developed reagents offer improved capabilities for zinc and copper imaging.
    • Further development in probe design will enhance our understanding of metal-related biological processes.