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Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
Published on: February 5, 2018
Rapid assembly of branched mannose oligosaccharides through consecutive regioselective glycosylation: A convergent
Bo Meng1, Jun Wang1, Qianli Wang1
1Omicron Biochemicals, Inc., 115 South Hill Street, South Bend, Indiana 46617-2701, USA.
Abstract:
A convergent and efficient strategy for the synthesis of high-mannose oligosaccharides is described wherein regioselective glycosylations between trichloroacetimidate donors and partially protected acceptors are employed to reduce the number of protection-deprotection steps. Two representative branched mannose oligosaccharides, a mannose heptasaccharide (Man7) and a mannose nonasaccharide (Man9) were constructed via (4+3) and (5+4) glycosylations, respectively. These mannose-containing oligosaccharides were obtained in nine steps in ~25% overall yield and >98% purity on 60-70 mg scales to demonstrate the effectiveness of the strategy.
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