Related Experiment Video
Updated: Feb 19, 2026
06:55
A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
Published on: November 7, 2018
9.5K
High Throughput Sequencing for Detection of Foodborne Pathogens
Camilla Sekse1, Arne Holst-Jensen1, Ulrich Dobrindt2
1Department of Animal Health and Food Safety, Norwegian Veterinary Institute, Oslo, Norway.
Frontiers in Microbiology
|November 7, 2017
Summary
High-throughput sequencing (HTS) offers advanced microbial typing for food safety. While promising for foodborne pathogen surveillance and outbreak investigations, challenges in data analysis and standardization remain.
Area of Science:
- Food safety science
- Microbial genomics
- Public health surveillance
Background:
- High-throughput sequencing (HTS) is a leading technology for microbial typing in clinical settings.
- Its application in foodborne pathogen monitoring and outbreak investigations is still developing.
Purpose of the Study:
- To review the literature on HTS for food pathogen surveillance.
- To assess the current status and future potential of HTS in improving food safety and reducing outbreak impacts.
- To identify challenges and opportunities for HTS implementation in food analysis.
Main Methods:
- Comprehensive literature review of published studies on HTS for bacterial, viral, and eukaryotic food pathogens.
- Analysis of HTS application in food surveillance, epidemiological, and outbreak investigations.
- Identification of technological and bioinformatic bottlenecks.
Main Results:
- HTS technology and bioinformatics advancements have outpaced data analysis capabilities.
- Obstacles include sample processing, nucleic acid extraction, standardized protocols, and curated reference databases.
- Despite challenges, significant progress in HTS capacity and application range has been observed over the last decade.
Conclusions:
- HTS holds immense potential for enhancing food safety and outbreak response.
- Addressing challenges in data interpretation, standardization, and database development is crucial.
- International collaborations are driving efforts to overcome obstacles and foster future progress in HTS applications for foodborne pathogen management.
