Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.9K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.9K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

5.6K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
5.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Atomic Evolution of Hydrogen Intercalation Wave Dynamics in Palladium Nanocrystals Revealed by Liquid-Phase Transmission Electron Microscopy.

Journal of the American Chemical Society·2026
Same author

Staging and defect-limited intercalation of FeCl<sub>3</sub> in graphite electrodes.

Nature communications·2026
Same author

Three-Dimensional Atomic Scale Insights into Unconventional Fragmentation of Two-Dimensional ReS<sub>2</sub> Monolayers into Molecular Clusters.

ACS nano·2026
Same author

Unsupervised Segmentation and Clustering Workflow for Efficient Processing of 4D-STEM and 5D-STEM Data.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same author

Nanocrystal Geometry Governs Phase Transformation Pathways in Palladium Hydride.

ACS nano·2026
Same author

Polyolefin blends with co-continuous architectures enabled by dynamic covalent crosslinking.

Science advances·2026
Same journal

Correction to: Accuracy of surface strain measurements from transmission electron microscopy images of nanoparticles.

Advanced structural and chemical imaging·2019
Same journal

Optimal principal component analysis of STEM XEDS spectrum images.

Advanced structural and chemical imaging·2019
Same journal

Protein structural biology using cell-free platform from wheat germ.

Advanced structural and chemical imaging·2018
Same journal

Multiscale higher-order TV operators for L1 regularization.

Advanced structural and chemical imaging·2018
Same journal

An open-source software ecosystem for the interactive exploration of ultrafast electron scattering data.

Advanced structural and chemical imaging·2018
Same journal

Sub-Ångstrom electric field measurements on a universal detector in a scanning transmission electron microscope.

Advanced structural and chemical imaging·2018
See all related articles

Related Experiment Video

Updated: Feb 19, 2026

Author Spotlight: Enhancing Cryo-Electron Microscopy by Automated Data Collection and Analysis Techniques
07:52

Author Spotlight: Enhancing Cryo-Electron Microscopy by Automated Data Collection and Analysis Techniques

Published on: December 1, 2023

1.5K

A streaming multi-GPU implementation of image simulation algorithms for scanning transmission electron microscopy.

Alan Pryor1, Colin Ophus2, Jianwei Miao1

  • 1Department of Physics and Astronomy and California NanoSystems Institute, University of California at Los Angeles, Los Angeles, CA 90095 USA.

Advanced Structural and Chemical Imaging
|November 7, 2017
PubMed
Summary
This summary is machine-generated.

This study introduces Prismatic, a software package accelerating scanning transmission electron microscopy (STEM) simulations using the PRISM method. It achieves significant speedups for atomic-resolution imaging and electron tomography, making complex analyses more accessible.

Keywords:
Atomic electron tomographyCUDAElectron scatteringGPUHigh performance computingImaging simulationMultislicePRISMScanning transmission electron microscopy

More Related Videos

Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management
10:23

Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management

Published on: June 23, 2023

3.6K
Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
08:46

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

10.5K

Related Experiment Videos

Last Updated: Feb 19, 2026

Author Spotlight: Enhancing Cryo-Electron Microscopy by Automated Data Collection and Analysis Techniques
07:52

Author Spotlight: Enhancing Cryo-Electron Microscopy by Automated Data Collection and Analysis Techniques

Published on: December 1, 2023

1.5K
Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management
10:23

Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management

Published on: June 23, 2023

3.6K
Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
08:46

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

10.5K

Area of Science:

  • Materials Science
  • Physics
  • Computational Science

Background:

  • Traditional scanning transmission electron microscopy (STEM) simulations are computationally intensive.
  • The plane-wave reciprocal-space interpolated scattering matrix (PRISM) method offers potential for faster simulations.
  • Accelerated STEM simulations are crucial for advanced imaging techniques like atomic electron tomography.

Purpose of the Study:

  • To develop and present Prismatic, a parallelized software package for STEM image simulations.
  • To implement both PRISM and multislice methods within the Prismatic package.
  • To demonstrate the computational speedups and applications of Prismatic in atomic-resolution imaging.

Main Methods:

  • Parallelized computation utilizing CUDA-enabled GPUs and multicore processors.
  • Implementation of the PRISM method for accelerated STEM simulations.
  • Implementation of the traditional multislice method for comparison and broader applicability.

Main Results:

  • Prismatic achieves up to 1000x acceleration for PRISM simulations and 15x for multislice simulations on a 4-GPU machine compared to traditional methods.
  • The software enables rapid computation of images for atomic electron tomography, integrating it into reconstruction pipelines.
  • Negligible loss of accuracy is observed with the PRISM method implemented in Prismatic.

Conclusions:

  • Prismatic significantly accelerates STEM image simulations, enhancing computational efficiency.
  • The software facilitates advanced applications such as atomic electron tomography by providing timely image data.
  • Prismatic is available as an open-source CUDA/C++ package with a GUI and a Python package (PyPrismatic).