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Related Experiment Video

Updated: Feb 19, 2026

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
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Murine In vitro Memory T Cell Differentiation.

Myoungjoo V Kim1, Weiming Ouyang1, Will Liao1

  • 1Department of Immunobiology, Yale University School of Medicine, New Haven, USA.

Bio-Protocol
|November 7, 2017
PubMed
Summary
This summary is machine-generated.

Generating large numbers of memory T cells in vitro is crucial for studying their function. This protocol enables the production of antigen-specific CD44hiCD62Lhi memory T cells, overcoming in vivo limitations.

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Area of Science:

  • Immunology
  • Cellular immunology
  • T cell biology

Background:

  • Naïve CD8+ T cells differentiate into effector and memory cells after pathogen encounter.
  • Memory T cells are vital for robust secondary infection responses.
  • Low in vivo frequency of memory T cells hinders functional studies.

Purpose of the Study:

  • To describe a detailed protocol for in vitro memory T cell differentiation.
  • To generate a large number of antigen-specific memory T cells.
  • To facilitate the investigation of memory T cell migration and genetic regulation.

Main Methods:

  • Detailed protocol for memory T cell differentiation.
  • Generation of CD44hiCD62Lhi antigen-specific memory T cells.
  • In vitro culture techniques.

Main Results:

  • Successful generation of large numbers of memory T cells in vitro.
  • Production of CD44hiCD62Lhi phenotype memory T cells.
  • Enabling further functional studies of memory T cells.

Conclusions:

  • This protocol provides a method to overcome the low frequency of memory T cells in vivo.
  • Facilitates research into memory T cell function, migration, and genetic regulation.
  • A valuable tool for immunological research.