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Updated: Feb 19, 2026

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
Published on: January 6, 2016
Uptake Assay for Radiolabeled Peptides in Yeast
Melinda Hauser1, Houjian Cai2, Fred Naider3,4
1Department of Microbiology, University of Tennessee, Knoxville, USA.
Abstract:
We describe an assay for measuring the uptake of radioactive peptides into the yeast Saccharomyces cerevisiae. The methods presented here can be adapted to measure a variety of substrates transported into any bacterial or fungal cell via specific carrier-mediated systems.
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