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Lymphocyte Isolation, Th17 Cell Differentiation, Activation, and Staining

Pawan Kumar1, Jay K Kolls1

  • 1Richard King Mellon Foundation Institute for Pediatric Research, Children's Hospital of Pittsburgh of UPMC, University of Pittsburgh, Pittsburgh, PA, USA.

Bio-Protocol
|November 7, 2017
PubMed

In vitro Th17 (α, β T helper cell which produce IL-17A, IL-17F and IL-22) differentiation has been routinely used for functional T cells studies. Here we describe a method for Th17 cell differentiation.

Keywords:
FACSIL-17Th17

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