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Related Experiment Video

Updated: Feb 19, 2026

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
12:59

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 26, 2013

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Lymphocyte Isolation, Th17 Cell Differentiation, Activation, and Staining.

Pawan Kumar1, Jay K Kolls1

  • 1Richard King Mellon Foundation Institute for Pediatric Research, Children's Hospital of Pittsburgh of UPMC, University of Pittsburgh, Pittsburgh, PA, USA.

Bio-Protocol
|November 7, 2017
PubMed
Summary

This study details a new method for inducing T helper 17 (Th17) cell differentiation in vitro. This protocol aids in functional T cell research by providing a reliable way to generate these important immune cells.

Keywords:
FACSIL-17Th17

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Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • T helper 17 (Th17) cells are crucial immune cells.
  • Th17 cells produce cytokines like IL-17A, IL-17F, and IL-22.
  • In vitro Th17 differentiation is vital for studying T cell function.

Purpose of the Study:

  • To describe a novel method for Th17 cell differentiation.
  • To provide a reliable protocol for researchers studying Th17 cells.

Main Methods:

  • In vitro cell culture techniques.
  • Specific cytokine and cellular environment manipulation for differentiation induction.

Main Results:

  • Successful differentiation of Th17 cells was achieved.
  • The described method provides a reproducible approach.

Conclusions:

  • The presented method facilitates Th17 cell differentiation.
  • This protocol supports functional T cell studies.