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Turbidimetric latex immunoassay of placental lactogen on microtiter plates
D Collet-Cassart1, J N Limet, L Van Krieken
1Unit of Experimental Medicine, International Institute of Cellular and Molecular Pathology, Brussels, Belgium.
Clinical Chemistry
|January 1, 1989
Abstract:
In this latex immunoassay for human placental lactogen, microtiter plates are used as the reaction vessel and the absorbance at 405 nm is measured to quantify the reactions. This 30-min assay necessitates only one serum dilution and two pipetting steps. The calibration curve extends from 0.5 to 15 mg/L. CVs range from 4.2% to 7.0% for within-run determination and from 7.0% to 11.2% for between-run determinations. A correlation coefficient of 0.949 was obtained for 84 sera when the method was compared with a commercial radioimmunoassay.