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Human cell lines expressing intestinal alkaline phosphatase.

F J Benham, H Harris

    Proceedings of the National Academy of Sciences of the United States of America
    |August 1, 1979
    PubMed
    Summary
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    Human alkaline phosphatase (ALP) is determined by at least three genetic loci. Studies on cell line D98/AH-2 reveal intestinal ALP, suggesting a loss of placental ALP expression in this cell line and its derivatives.

    Area of Science:

    • Biochemistry
    • Genetics
    • Cell Biology

    Background:

    • Human alkaline phosphatases (ALPs) are enzymes encoded by multiple genetic loci, with distinct forms found in the placenta, intestine, and liver/bone/kidney.
    • Understanding the expression patterns of these isoenzymes is crucial for cellular and genetic studies.

    Purpose of the Study:

    • To characterize the alkaline phosphatase (ALP) isoenzyme present in the D98/AH-2 cell line.
    • To investigate the expression of placental and intestinal ALP in a series of related cell lines (Detroit 98, D98/S, D98/AH-R) believed to be of HeLa origin.

    Main Methods:

    • Biochemical characterization of ALP in D98/AH-2 cells using inhibition assays and thermostability studies.
    • Electrophoretic analysis to determine the type of ALP present.

    Related Experiment Videos

  • Comparative analysis of ALP expression across different cell lines.

    • Main Results:

    • The alkaline phosphatase (ALP) in D98/AH-2 cells was identified as intestinal type, closely resembling the fetal intestinal form.
    • Intestinal ALP was detected in related cell lines Detroit 98, D98/S, and D98/AH-R.
    • No placental alkaline phosphatase (PLAP) was detected in any of the studied Detroit-98 derivative cell lines.

    Conclusions:

    • The D98/AH-2 cell line and its derivatives express intestinal alkaline phosphatase (ALP) but have lost the expression of placental alkaline phosphatase (PLAP).
    • This loss of PLAP expression likely occurred early in the lineage of these cell lines, possibly before their divergence from other HeLa cell lines.
    • The findings provide insights into the genetic regulation and expression variability of human alkaline phosphatase isoenzymes in cell lines.