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Alteration in Ikaros expression promotes B-1 cell differentiation into phagocytes
Vivian Cristina de Oliveira1, Ana Clara Pires Sodré1, Caio Perez Gomes2
1Disciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Brazil.
Immunobiology
|November 7, 2017
Summary
Ikaros transcription factor isoforms regulate B-1 cell plasticity. Low expression and abnormal localization of Ikaros in B-1 cell-derived phagocytes suggest a role in their differentiation.
Area of Science:
- Immunology
- Molecular Biology
- Hematopoiesis
Background:
- Ikaros is a key transcription factor regulating lymphocyte development.
- Distinct Ikaros isoforms control lymphoid and myeloid lineage differentiation.
- Aberrant Ikaros expression is linked to hematological disorders.
Purpose of the Study:
- To investigate the role of Ikaros in B-1 cell plasticity.
- To examine Ikaros expression and localization in B-1 cell-derived phagocytes (B-1CDP).
Main Methods:
- Analysis of Ikaros expression and isoform profiles in B-1 cells and B-1CDP.
- Immunocytochemistry to determine Ikaros protein localization.
Main Results:
- B-1CDP cells exhibit low Ikaros expression.
- Ikaros protein is abnormally localized in the cytoplasm of B-1CDP cells.
- B-1 cells express different Ikaros isoforms, with reduced active forms in B-1CDP.
Conclusions:
- Ikaros plays a role in controlling B-1 cell plasticity.
- Altered Ikaros expression and localization may drive B-1 cell differentiation into phagocytes.
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