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Updated: Feb 19, 2026
The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells
Published on: April 14, 2013
Default Patterning Produces Pan-cortical Glutamatergic and CGE/LGE-like GABAergic Neurons from Human Pluripotent Stem
Crina M Floruta1, Ruofei Du2, Huining Kang3
1Department of Neurosciences, University of New Mexico-Health Science Center, Albuquerque, NM 87131, USA.
Abstract:
Default differentiation of human pluripotent stem cells has been promoted as a model of cortical development. In this study, a developmental transcriptome analysis of default-differentiated hPSNs revealed a gene expression program resembling in vivo CGE/LGE subpallial domains and GABAergic signaling. A combination of bioinformatic, functional, and immunocytochemical analysis further revealed that hPSNs consist of both cortical glutamatergic and CGE-like GABAergic neurons. This study provides a comprehensive characterization of the heterogeneous group of neurons produced by default differentiation and insight into future directed differentiation strategies.
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