Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Source And Potency Of Stem Cells01:27

Source And Potency Of Stem Cells

6.4K
Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...
6.4K
Formation of Muscle Fibers from Myoblasts01:13

Formation of Muscle Fibers from Myoblasts

6.1K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
6.1K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

5.7K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
5.7K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

28.2K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.2K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.7K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.7K
Satellite Stem Cells and Muscular Dystrophy01:21

Satellite Stem Cells and Muscular Dystrophy

2.4K
Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
2.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prostate-specific Antigen Nadir and Time to Nadir Predict Recurrence in Postprostatectomy Patients Treated with Salvage Radiotherapy Without Androgen Deprivation Therapy.

European urology oncology·2025
Same author

Development of an AAV-delivered microRNA gene therapy for myotonic dystrophy type 1.

Molecular therapy : the journal of the American Society of Gene Therapy·2025
Same author

Predictors of toxicities and oncological outcomes following postoperative ablative radiotherapy (POPART) for biochemical recurrence.

Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology·2025
Same author

Preclinical quality, safety, and efficacy of a CGMP iPSC-derived myogenic progenitor product for the treatment of muscular dystrophies.

Molecular therapy : the journal of the American Society of Gene Therapy·2025
Same author

PAX translocations remodel mitochondrial metabolism through altered leucine usage in rhabdomyosarcoma.

Cell·2025
Same author

Analysis of patients with locally advanced rectal cancer given neoadjuvant radiochemotherapy with or without RT dose intensification: A multicenter retrospective study - ATLANTIS part I.

Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology·2025

Related Experiment Video

Updated: Feb 19, 2026

Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System
07:09

Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System

Published on: May 10, 2020

5.4K

Myogenic progenitor specification from pluripotent stem cells.

Alessandro Magli1, Rita R C Perlingeiro1

  • 1Lillehei Heart Institute, Department of Medicine, University of Minnesota, Minneapolis, MN, USA.

Seminars in Cell & Developmental Biology
|November 7, 2017
PubMed
Summary
This summary is machine-generated.

Pluripotent stem cells are key for studying development and disease. This review explores how understanding embryonic skeletal muscle development aids in generating these cells from stem cells for potential therapies.

Keywords:
BMP inhibitorCell therapyDisease modelingES cellsGSK3 inhibitorMesoderm specificationMuscle developmentMuscle regenerationMyf5MyoDMyogenesisMyogenic progenitorParaxial mesodermPax3Pax7Pluripotent stem cellsSomiteTGFβ inhibitorWNT activationiPS cells

More Related Videos

Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration
10:03

Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration

Published on: January 20, 2014

10.1K
Generation of Myospheres From hESCs by Epigenetic Reprogramming
09:32

Generation of Myospheres From hESCs by Epigenetic Reprogramming

Published on: June 21, 2014

8.3K

Related Experiment Videos

Last Updated: Feb 19, 2026

Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System
07:09

Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System

Published on: May 10, 2020

5.4K
Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration
10:03

Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration

Published on: January 20, 2014

10.1K
Generation of Myospheres From hESCs by Epigenetic Reprogramming
09:32

Generation of Myospheres From hESCs by Epigenetic Reprogramming

Published on: June 21, 2014

8.3K

Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Regenerative Medicine

Background:

  • Pluripotent stem cells are valuable for research and cell therapy.
  • Understanding skeletal muscle development is crucial for generating myogenic cells.
  • Advances in stem cell science enable in vitro recapitulation of developmental processes.

Purpose of the Study:

  • To review molecular mechanisms of embryonic skeletal myogenesis.
  • To explain how this knowledge informs specification from pluripotent stem cells.
  • To discuss limitations and future applications of stem cell-derived myogenic cells.

Main Methods:

  • Review of existing literature on skeletal myogenesis and stem cell differentiation.
  • Analysis of molecular pathways involved in embryonic muscle development.
  • Evaluation of current in vitro methods for myogenic lineage specification.

Main Results:

  • Detailed insights into molecular mechanisms driving skeletal muscle commitment in embryos.
  • Demonstration of how embryonic knowledge facilitates in vitro myogenic lineage specification.
  • Identification of challenges in fully recapitulating skeletal myogenesis in vitro.

Conclusions:

  • Knowledge of embryonic skeletal myogenesis is instrumental for deriving myogenic cells from pluripotent stem cells.
  • Current methods show promise but face limitations in fully replicating in vivo myogenesis.
  • Future applications include cell therapy and disease modeling using stem cell-derived myogenic progenitors.