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Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Related Experiment Video

Updated: Feb 19, 2026

Author Spotlight: Exploring Cellular Processes by Modeling Ligands in Cryo-EM Maps

Author Spotlight: Exploring Cellular Processes by Modeling Ligands in Cryo-EM Maps

Published on: July 19, 2024

CryoEM-based hybrid modeling approaches for structure determination.

C Keith Cassidy¹, Benjamin A Himes², Zaida Luthey-Schulten³

¹Department of Physics, Beckman Institute, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Current Opinion in Microbiology

|November 7, 2017

Summary

Recent cryo-electron microscopy (cryoEM) advances enable high-resolution imaging of biological specimens. Hybrid modeling methods are now used to build accurate atomic models from cryoEM data, offering new structural insights.

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Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Published on: January 9, 2015

Cryo-EM and Single-Particle Analysis with Scipion

Cryo-EM and Single-Particle Analysis with Scipion

Published on: May 29, 2021

Related Experiment Videos

Last Updated: Feb 19, 2026

Author Spotlight: Exploring Cellular Processes by Modeling Ligands in Cryo-EM Maps

Author Spotlight: Exploring Cellular Processes by Modeling Ligands in Cryo-EM Maps

Published on: July 19, 2024

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Published on: January 9, 2015

Cryo-EM and Single-Particle Analysis with Scipion

Cryo-EM and Single-Particle Analysis with Scipion

Published on: May 29, 2021

Area of Science:

Structural biology
Biophysics
Computational biology

Background:

Cryo-electron microscopy (cryoEM) has achieved significant resolution improvements.
High-fidelity atomistic models are crucial for understanding biological mechanisms.

Purpose of the Study:

To review cryoEM-based hybrid modeling techniques.
To provide an overview of computational tools and strategies.
To highlight recent applications of these methods.

Main Methods:

Hybrid modeling approaches integrating experimental data.
Computational tools for model building and refinement.
Analysis of cryoEM data at various scales.

Main Results:

CryoEM enables detailed structural and mechanistic insights.
Hybrid modeling facilitates high-fidelity atomistic model construction.
Diverse applications showcase the power of these integrated methods.

Conclusions:

CryoEM-based hybrid modeling is a powerful approach.
Advances in computational tools enhance model accuracy.
This methodology provides significant structural and mechanistic understanding.