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The clinical significance and biological function of interferon regulatory factor 1 in cholangiocarcinoma

Peiqi Wan1, Junhong Zhang2, Qiang Du3

  • 1Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA; Department of infectious diseases, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, PR China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|November 7, 2017
PubMed

Insights

Interferon regulatory factor 1 (IRF1) is downregulated in cholangiocarcinoma, correlating with poor prognosis. Restoring IRF1 suppresses tumor growth, migration, and invasion, supporting its role as a tumor suppressor.

Area of Science:

  • Oncology
  • Molecular Biology
  • Hepatobiliary Surgery

Background:

  • Interferon regulatory factor 1 (IRF1) is implicated as a tumor suppressor in various human cancers.
  • The specific role and clinical significance of IRF1 in cholangiocarcinoma remain largely uncharacterized.

Purpose of the Study:

  • To investigate the expression levels, clinical significance, and biological functions of IRF1 in cholangiocarcinoma.

Main Methods:

  • Quantitative analysis of IRF1 mRNA and protein expression in cholangiocarcinoma tissues and cell lines versus normal controls.
  • Correlation analysis between IRF1 expression and clinicopathological parameters.
  • Functional assays to assess the impact of IRF1 up-regulation on cholangiocarcinoma cell proliferation, migration, invasion, cell cycle, and apoptosis.

Main Results:

  • IRF1 mRNA and protein expression were significantly decreased in cholangiocarcinoma tissues and cell lines.
  • Low IRF1 protein expression correlated with advanced tumor stage, larger tumor size, vascular invasion, and metastasis.
  • IRF1 served as an independent poor prognostic factor for cholangiocarcinoma patients.
  • IRF1 up-regulation inhibited cholangiocarcinoma cell proliferation, migration, and invasion, and induced cell cycle arrest without affecting apoptosis.

Conclusions:

  • IRF1 is frequently downregulated in cholangiocarcinoma, associated with aggressive disease and poor outcomes.
  • IRF1 functions as a tumor suppressor by inhibiting proliferation, migration, invasion, and cell cycle progression in cholangiocarcinoma.
Keywords:
BiomarkerCholangiocarcinomaIRF1Prognosis

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