Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pharmacokinetics in Geriatric Patients: Effect of Age on Drug Metabolism01:18

Pharmacokinetics in Geriatric Patients: Effect of Age on Drug Metabolism

242
Geriatric patients show significant variation in how their bodies process medications, which can change how effective and safe treatments are. The liver is the primary organ where drug metabolism occurs, involving two main types of chemical reactions: phase I and II. Phase I metabolism is driven by the cytochrome P450 enzyme system, which includes key types such as CYP3A, CYP2D6, and CYP2C9. Research indicates that while aging doesn't notably alter the levels or activity of these enzymes, it...
242
Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

19.1K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
19.1K
Pharmacodynamics in Geriatric Patients: Effects of Age01:27

Pharmacodynamics in Geriatric Patients: Effects of Age

253
Age-related pharmacokinetic changes are extensively documented, but understanding age-related pharmacodynamic alterations is relatively limited. This knowledge gap can be partly attributed to the complexity of developing appropriate measures of drug responses compared to bioanalytical methods for determining drug concentrations.Most information regarding age-related differences in human pharmacodynamics originates from cross-sectional studies. However, these studies assume that observed mean...
253
Abnormal Proliferation02:23

Abnormal Proliferation

5.3K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficacy Analysis of Paiteling in Treating Persistent High-Risk Human Papillomavirus after Cervical Cancer Surgery.

Journal of visualized experiments : JoVE·2026
Same author

Clinical characteristics and comorbidities of sleep apnea in the older and oldest-old inpatients.

Lung India : official organ of Indian Chest Society·2026
Same author

Development of Chloroplast Microsatellite Markers and Assessment of Genetic Diversity and Population Structure of <i>Sophora tonkinensis</i> Gagnep. in Southwestern China.

Current issues in molecular biology·2026
Same author

Surface Association of Flaxseed Oil on Cassava Starch Granules via Prolonged Mild Thermal Treatment: Structural, Pasting, Textural, and Emulsifying Properties.

Foods (Basel, Switzerland)·2026
Same author

Quality improvement strategies for preventing tracheostomy-related pressure injuries in children: a systematic review and meta-analysis.

Frontiers in pediatrics·2026
Same author

A Case Report of Shwachman-Diamond Syndrome Caused by Heterozygous Variants in the EFL1 Gene and Literature Review.

Molecular genetics & genomic medicine·2026

Related Experiment Video

Updated: Feb 19, 2026

An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants
07:44

An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants

Published on: May 22, 2020

28.5K

MeCP2 mediated dysfunction in senescent EPCs.

Chunli Wang1, Fei Wang1, Zhen Li1

  • 1Department of Geriatrics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Oncotarget
|November 8, 2017
PubMed
Summary
This summary is machine-generated.

Aging impairs endothelial progenitor cells (EPCs). Methyl CpG binding protein 2 (MeCP2) exacerbates this by inhibiting SIRT1, reducing EPC function. Silencing MeCP2 improves senescent EPCs.

Keywords:
EPCsGerotargetMeCP2SIRT1senescence

More Related Videos

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence
13:59

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

Published on: August 12, 2018

8.6K
Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry
08:52

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Published on: April 6, 2022

4.1K

Related Experiment Videos

Last Updated: Feb 19, 2026

An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants
07:44

An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants

Published on: May 22, 2020

28.5K
A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence
13:59

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

Published on: August 12, 2018

8.6K
Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry
08:52

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Published on: April 6, 2022

4.1K

Area of Science:

  • Cell Biology
  • Epigenetics
  • Aging Research

Background:

  • Aging endothelial progenitor cells (EPCs) show impaired function.
  • Sirtuin 1 (SIRT1) is crucial for maintaining EPC function.
  • Methyl CpG binding protein 2 (MeCP2) is an epigenetic regulator involved in cellular processes like senescence.

Purpose of the Study:

  • To investigate the impact of MeCP2 on the functional activities of senescent EPCs.
  • To elucidate the underlying molecular mechanisms involving SIRT1.

Main Methods:

  • Western blot and real-time PCR to assess protein and mRNA levels.
  • Adenoviral vector transduction for gene overexpression and silencing.
  • Co-transfection experiments to study gene interactions.

Main Results:

  • MeCP2 expression was upregulated, while SIRT1 was downregulated in senescent EPCs.
  • Overexpression of MeCP2 reduced EPC function; MeCP2 silencing improved it.
  • MeCP2 modulated SIRT1 expression, and SIRT1 reversed MeCP2's effects on EPCs.

Conclusions:

  • MeCP2 exacerbates senescence in EPCs by inhibiting SIRT1.
  • Targeting MeCP2 may offer a therapeutic strategy for age-related vascular dysfunction.