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Related Experiment Videos

Interphotoreceptor retinoid-binding protein. Gene characterization, protein repeat structure, and its evolution.

D E Borst1, T M Redmond, J E Elser

  • 1Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, Bethesda, Maryland 20892.

The Journal of Biological Chemistry
|January 15, 1989
PubMed
Summary

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Researchers cloned the bovine interphotoreceptor retinoid-binding protein (IRBP) gene, revealing a unique structure with four exons and three introns. This finding offers insights into the evolution of the IRBP gene and its protein repeats.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • The interphotoreceptor retinoid-binding protein (IRBP) plays a crucial role in vision.
  • Understanding the genetic basis of IRBP is essential for comprehending its function and evolution.

Purpose of the Study:

  • To clone and determine the nucleotide sequence of the bovine IRBP gene.
  • To analyze the gene structure, including exons and introns.
  • To investigate the protein's repeat structure and its correlation with gene organization.

Main Methods:

  • Gene cloning and nucleotide sequencing of the bovine IRBP gene.
  • mRNA and protein analysis to determine size and sequence.
  • Protein fragmentation (tryptic and cyanogen bromide) and sequence analysis.

Related Experiment Videos

  • Comparison of bovine IRBP gene sequence with human IRBP gene sequences.

    • Main Results:

    • The bovine IRBP gene is approximately 11.6 kb, containing four exons and three introns.
    • The gene transcribes to a 6.4 kb mRNA and translates to a 145,000-dalton protein.
    • The protein exhibits a 4-fold repeat structure with significant sequence identity between repeats.
    • All three introns are located within the fourth protein repeat, and exon sizes vary significantly.
    • A sequence similarity was found between a bovine IRBP intron and the human IRBP gene.

    Conclusions:

    • The bovine IRBP gene possesses an unexpected structure with introns clustered in the final protein repeat.
    • The identified protein repeat structure suggests potential gene duplication events during evolution.
    • A model for the evolution of the IRBP gene is proposed based on the observed gene and protein structures.