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Related Concept Videos

High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

3.2K
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
3.2K
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

3.7K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
3.7K
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

1.6K
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
1.6K
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

1.8K
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
1.8K
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

1.3K
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
1.3K
Gas Chromatography: Sample Injection Systems01:08

Gas Chromatography: Sample Injection Systems

1.6K
In gas chromatography, the sample is introduced as a vapor plug into the carrier gas stream for high efficiency and resolution. A microsyringe injects the sample solution into a heated sample port, vaporizing it and mixing it with the carrier gas. This process is important to ensure the sample is properly prepared for analysis. Thermally sensitive samples can be injected directly into the column and volatilized by slowly increasing the column temperature.
Two primary injection methods are used...
1.6K

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Related Experiment Video

Updated: Feb 19, 2026

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
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Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

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Miniaturized high-performance liquid chromatography instrumentation.

Kyle B Lynch1, Apeng Chen1, Shaorong Liu1

  • 1Department of Chemistry and Biochemistry, University of Oklahoma, USA.

Talanta
|November 8, 2017
PubMed
Summary
This summary is machine-generated.

Miniaturized high-performance liquid chromatography (HPLC) systems offer potential for rapid, point-of-care analyses. This review covers recent advancements in miniaturized HPLC components, systems, and applications.

Keywords:
Electroosmotic pumpGradient generatorHigh performance liquid chromatographyMiniaturizedNanoflow

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Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
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Last Updated: Feb 19, 2026

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Area of Science:

  • Analytical Chemistry
  • Instrumental Science
  • Biotechnology

Background:

  • Miniaturized high-performance liquid chromatography (HPLC) is gaining traction for its efficiency.
  • Key applications include high-throughput analyses and point-of-care diagnostics.
  • Advancements in miniaturization are crucial for portable and accessible analytical tools.

Purpose of the Study:

  • To review recent progress in miniaturized HPLC system development.
  • To analyze the advantages and disadvantages of miniaturized HPLC components.
  • To discuss current applications and future trends in miniaturized HPLC technology.

Main Methods:

  • Comprehensive literature review of recent advancements in miniaturized HPLC.
  • Analysis of key components: pumps, injectors, columns, and detectors.
  • Evaluation of representative miniaturized HPLC systems and their performance.

Main Results:

  • Significant progress in developing compact and efficient HPLC components.
  • Emergence of diverse miniaturized HPLC systems tailored for specific applications.
  • Demonstrated feasibility of miniaturized HPLC for various analytical tasks.

Conclusions:

  • Miniaturized HPLC technology is rapidly evolving, offering enhanced portability and performance.
  • Continued innovation in components and system integration will drive broader adoption.
  • Future developments are expected to expand applications in fields like clinical diagnostics and environmental monitoring.