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Updated: Feb 19, 2026

Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection
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Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection

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A temperature-sensitive replicon enables efficient gene inactivation in Pseudomonas aeruginosa

Ramadevi Prathapam1, Tsuyoshi Uehara1

  • 1Infectious Diseases Area, Novartis Institutes for BioMedical Research, 5300 Chiron Way, Emeryville, CA 94608, United States.

Journal of Microbiological Methods
|November 8, 2017
PubMed

Keywords:
Gene inactivationPseudomonas aeruginosaTemperature-sensitive replicon

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