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Selective nitric oxide synthase inhibitor promotes bone healing.

Ali Reza Farhad1, Sayed Mohammad Razavi2, Ali Reza Rozati3

  • 1Department of Endodontics, Dental Research Center, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.

Dental Research Journal
|November 8, 2017
PubMed
Summary

Aminoguanidine (AG), an inducible nitric oxide synthase (iNOS) inhibitor, significantly enhanced bone healing rates in rats. Local AG application accelerated new mature bone formation, suggesting its potential in clinical bone regeneration.

Keywords:
Aminoguanidinebonehealingnitric oxidenitric oxide synthase

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Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Orthopedics

Background:

  • Nitric oxide (NO) plays a crucial role in bone metabolism and wound healing processes.
  • Selective inhibition of inducible nitric oxide synthase (iNOS) is explored for therapeutic applications.
  • Aminoguanidine (AG) is a known selective inhibitor of iNOS.

Purpose of the Study:

  • To evaluate the local impact of aminoguanidine (AG) on the rate of bone healing.
  • To assess the efficacy of AG in promoting new bone formation.
  • To compare the effects of AG with control and placebo treatments.

Main Methods:

  • An experimental interventional study involving 36 rats with femur bone defects.
  • Random assignment into three groups: control (empty defect), placebo (gel), and AG (AG gel).
  • Histological and histomorphometric analyses to assess new bone formation and healing scores.

Main Results:

  • The AG group exhibited a significantly higher mean healing score compared to control and placebo groups (P=0.036).
  • A significantly greater percentage of new mature (lamellar) bone was observed in the AG group (P=0.008).
  • Histomorphometric analysis confirmed accelerated bone regeneration with AG application.

Conclusions:

  • Local application of aminoguanidine (AG) accelerates the rate of bone healing.
  • Selective iNOS inhibitors like AG show potential as adjuncts for promoting local bone formation in clinical settings.
  • This study supports the therapeutic potential of iNOS inhibition in bone regeneration.