Related Experiment Video
Updated: Feb 19, 2026

A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells
Published on: September 28, 2019
Registration of Partially Focused Images for 2D and 3D Reconstruction of Oversized Samples
Dalibor Martišek1, Hana Druckmüllerová1
1Faculty of Mechanical Engineering, Institute of Mathematics, Brno University of Technology, Technická 2, 616 69 Brno, Czech Republic.
Abstract:
Methods of fracture surface 3D reconstruction from a series of partially focused images acquired in a small field of view (e.g., by confocal microscope or CCD camera) are well known. In this case, projection rays can be considered parallel and recorded images do not differ in any geometrical transformation from each other. In the case of larger samples (oversized for microscope or CCD camera), it is necessary to use a wider viewing field (e.g., standard cameras); taken images primarily differ in scaling but may also differ in shifting and rotation. These images cannot be used for reconstruction directly; they must be registered; that is, we must determine all transformations in which the images differ and eliminate their effects. There are several ways to do this. This paper deals with the registration based on phase correlation.

