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Effects of Chlamydia trachomatis infection on sperm chromatin condensation and DNA integrity

L Dehghan Marvast1, A R Talebi2, J Ghasemzadeh2

  • 1Department of Human Metabolism, Academic Unit of Reproductive and Developmental Medicine, The University of Sheffield, Sheffield, UK.

Andrologia
|November 8, 2017
PubMed

Insights

This study found no link between Chlamydia trachomatis infection and sperm DNA integrity in infertile Iranian men. Sperm DNA fragmentation was associated with immotile sperm, not C. trachomatis.

Area of Science:

  • Reproductive Medicine
  • Infectious Diseases
  • Genetics

Background:

  • Chlamydia trachomatis infection is a potential cause of male infertility.
  • Sperm chromatin and DNA integrity are crucial for male fertility.
  • The relationship between C. trachomatis and sperm DNA integrity requires further investigation.

Purpose of the Study:

  • To investigate the association between Chlamydia trachomatis infection and sperm chromatin/DNA integrity in infertile men from Iran.

Main Methods:

  • Collected blood, semen, and urine samples from 250 infertile men.
  • Performed serological tests for C. trachomatis antibodies.
  • Detected C. trachomatis DNA in urine and semen samples.
  • Assessed sperm DNA fragmentation and chromatin abnormalities using multiple assays (aniline blue, chromomycin A3, acridine orange, TUNEL).

Main Results:

  • No evidence of C. trachomatis infection was detected in semen samples.
  • Sperm DNA fragmentation and chromatin abnormalities did not correlate with C. trachomatis diagnosis.
  • Increased DNA fragmentation was linked to higher percentages of immotile sperm and lower semen volume and normal morphology.
  • Only chromatin protamination correlated with male age.

Conclusions:

  • C. trachomatis infection does not appear to directly impact sperm DNA integrity in this cohort of infertile men.
  • Sperm DNA fragmentation is associated with sperm motility and semen quality parameters.
  • Male age is a factor in sperm chromatin protamination.
Keywords:
C. trachomatisDNAinfertilitysperm chromatin

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