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Updated: Feb 19, 2026
Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
Published on: April 4, 2025
Tetrazolylpyrene unnatural nucleoside as a human telomeric multimeric G-quadruplex selective switch-on fluorescent
Subhendu Sekhar Bag1, Manoj Kumar Pradhan, Sangita Talukdar
1Bioorganic Chemistry Laboratory, Department of Chemistry, Indian Institute of Technology Guwahati, North Guwhati-781039, Assam, India. ssbag75@iitg.ernet.in.
Abstract:
We report herein the specific sensing of dimeric H45 G-quadruplex DNA via a fluorescence light-up response using fluorescent tetrazolylpyrene nucleoside (TzPyBDo) as a probe. The strong binding of the probe via an intercalative stacking interaction inside the connecting loop of two G-quadruplex units of H45 and the discrimination to other monomeric and long DNA duplexes are accompanied by a drastic enhancement of the emission intensity without compromising the conformation and stability.
