Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

1.4K
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
1.4K
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

1.9K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
1.9K
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.6K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.6K
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

1.9K
The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
1.9K
¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

1.9K
A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied...
1.9K
IR Spectrometers01:25

IR Spectrometers

2.7K
There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Specific Ion Effects of Chaotropic and Superchaotropic Anions Probed by Raman Hydration-Shell Spectroscopy.

Angewandte Chemie (International ed. in English)·2026
Same author

Tomographic Sparse View Selection Using the View Covariance Loss.

IEEE transactions on pattern analysis and machine intelligence·2025
Same author

XCal: model-based approach to X-ray CT spectral calibration.

Optics express·2025
Same author

A machine learning decision criterion for reducing scan time for hyperspectral neutron computed tomography systems.

Scientific reports·2024
Same author

Influence of H<sup>+</sup>, OH<sup>-</sup> and salts on hydrophobic self-assembly.

Chemical science·2024
Same author

Interfacial chemical reactivity enhancement.

The Journal of chemical physics·2024
Same journal

EXPRESS: Deterministic Compressed Sensing in Time-Domain Spectroscopy.

Applied spectroscopy·2026
Same journal

EXPRESS: Multi-Parameter Wavelength Characterization of Array Spectrometers Under Near-Limit Sampling Conditions.

Applied spectroscopy·2026
Same journal

EXPRESS: A Validated Reference Database for Twentieth-Century Cd-Based Pigments: Integrated Structural and Compositional Characterization.

Applied spectroscopy·2026
Same journal

EXPRESS: Two-Trace Two-Dimensional (2T2D-COS) in the Analysis of Brain Tissue Sample Preparation Method.

Applied spectroscopy·2026
Same journal

EXPRESS: Simplified Protocol for Analyzing Polarization Properties of Scanning Tunneling Microscope (STM) Light Emission Spectra at an Oblique Angle.

Applied spectroscopy·2026
Same journal

EXPRESS: Monitoring a Polyurethane Synthesis by Fiber-Coupled Attenuated Total Reflection Fourier Transform Infrared Spectroscopy and Multivariate Curve Resolution-Alternating Least Squares.

Applied spectroscopy·2026
See all related articles

Related Experiment Video

Updated: Feb 19, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

512

Binary Complementary Filters for Compressive Raman Spectroscopy.

Owen G Rehrauer1, Vu C Dinh2, Bharat R Mankani1

  • 11 8522 Department of Chemistry, Purdue University, West Lafayette, IN, USA.

Applied Spectroscopy
|November 8, 2017
PubMed
Summary
This summary is machine-generated.

The new OB-CD2 strategy enhances hyperspectral Raman spectroscopy by detecting all scattered photons, improving chemical analysis speed and accuracy. This method uses complementary filters and photon counting detectors for faster, more precise component identification.

Keywords:
Raman spectroscopychemometricscompressive sampling

More Related Videos

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

4.6K
Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional &#960;-conjugate Systems
09:57

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

7.7K

Related Experiment Videos

Last Updated: Feb 19, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

512
Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

4.6K
Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional &#960;-conjugate Systems
09:57

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

7.7K

Area of Science:

  • Spectroscopy
  • Optics
  • Chemical analysis

Background:

  • Optimized Binary Compressive Detection (OB-CD) allows rapid hyperspectral Raman analysis.
  • The original OB-CD strategy results in photon loss, limiting detection efficiency.

Purpose of the Study:

  • To introduce and validate the OB-CD2 strategy, a refinement of OB-CD.
  • To improve photon detection efficiency and accuracy in hyperspectral Raman spectroscopy.

Main Methods:

  • Developed OB-CD2 utilizing complementary binary optical filters and two photon-counting detectors.
  • Employed a novel optimization algorithm, holographic volume diffraction grating, and digital micromirror device (DMD).
  • Programmed DMD to selectively direct photons to detectors based on color.

Main Results:

  • OB-CD2 detects all collected Raman photons, unlike the original OB-CD.
  • Achieved more accurate estimation of Raman scattering rates for chemical components.
  • Demonstrated rapid chemical classification at speeds as fast as 3 μs per measurement.
  • Enabled collection of Raman images in under one second.

Conclusions:

  • The OB-CD2 strategy significantly enhances hyperspectral Raman spectroscopic analysis.
  • This method offers improved accuracy and speed for chemical identification and imaging.
  • OB-CD2 represents a substantial advancement in photon detection for spectroscopic applications.