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Updated: Feb 19, 2026
Cellular Toxicity of Nanogenomedicine in MCF-7 Cell Line: MTT assay
Published on: April 3, 2009
DNA methylation alterations induced by transient exposure of MCF-7 cells to maghemite nanoparticles
Raphael S Bonadio1, Ana Carolina Arcanjo1, Emilia Cd Lima2
1Genetics & Morphology Department, University of Brasilia, Brasília, Brazil.
Aim:
To evaluate the DNA methylation profile of MCF-7 cells during and after the treatment with maghemite nanoparticles (MNP-CIT).
Materials & Methods:
Noncytotoxic MNP-CIT concentrations and cell morphology were evaluated by standard methods. DNA methylation was assessed by whole genome bisulfite sequencing. DNA methyltransferase (DNMT) genes expression was analyzed by qRT-PCR.
Results:
A total of 30 and 60 µgFeml-1 MNP-CIT accumulated in cytoplasm but did not present cytotoxic effects. The overall percentage of DNA methylation was not affected, but 58 gene-associated regions underwent DNA methylation reprogramming, including genes related to cancer onset. DNMT transcript levels were also modulated.
Conclusion:
Transient exposure to MNP-CIT promoted epigenomic changes and altered the DNMT genes regulation in MCF-7 cells. These events should be considered for biomedical applications.
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