Melaminivora jejuensis sp. nov., isolated from Swinery waste

A sulfur-oxidizing bacterium, designated strain KBB12^T, was isolated from swinery waste collected in Jeju, Republic of Korea. The cells were Gram-stain-negative, flagellated and rod-shaped. Growth occurred at 15-45 °C (optimum, 30-37 °C), at pH 6-9 (optimum, pH 7.0) and in the presence of 0-1 % (w/v) NaCl. The major cellular fatty acids were summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c, C16 : 0 and C18 : 1ω7c. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phospholipid and an unidentified lipid. The major isoprenoid quinone was ubiquinone-8 (Q-8) and the DNA G+C content of the genomic DNA was 69.6 mol%. Phylogenetic analyses, based on 16S rRNA gene sequences, showed that the novel isolate belongs to the genus Melaminivora and was most closely related to Melaminivora alkalimesophila CY1^T (97.2 % similarity). The DNA-DNA relatedness values between strain KBB12^T and M. alkalimesophila DSM26005^T was 43.4±2.7 %. On the basis of phylogenetic and phenotypic evidence, it is proposed that strain KBB12^T represents a novel species of the genus Melaminivora, for which the name Melaminivora jejuensis sp. nov. is proposed. The type strain is KBB12^T (=KCTC 32230^T=JCM 18740^T).