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Updated: Feb 19, 2026

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Published on: June 27, 2022
Chemical Biology of H2S Signaling through Persulfidation
Milos R Filipovic1,2, Jasmina Zivanovic1,2, Beatriz Alvarez3
1Univeristy of Bordeaux, IBGC, UMR 5095 , F-33077 Bordeaux, France.
Abstract:
Signaling by H2S is proposed to occur via persulfidation, a posttranslational modification of cysteine residues (RSH) to persulfides (RSSH). Persulfidation provides a framework for understanding the physiological and pharmacological effects of H2S. Due to the inherent instability of persulfides, their chemistry is understudied. In this review, we discuss the biologically relevant chemistry of H2S and the enzymatic routes for its production and oxidation. We cover the chemical biology of persulfides and the chemical probes for detecting them. We conclude by discussing the roles ascribed to protein persulfidation in cell signaling pathways.
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