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Neural Lineage Progression Controlled by a Temporal Proliferation Program.

Shahrzad Bahrampour¹, Erika Gunnar¹, Carolin Jonsson¹

¹Department of Clinical and Experimental Medicine, Linkoping University, 58185 Linkoping, Sweden.

Developmental Cell

|November 8, 2017

Summary

This summary is machine-generated.

Stem cell differentiation involves down-regulating proliferation. This study identifies transcription factors controlling Drosophila neuroblast cell cycle exit and daughter cell proliferation transitions.

Keywords:

alternate daughter proliferation combinatorial codes neural lineage neural stem cell re-programing temporal control of proliferation

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Area of Science:

Developmental Biology
Stem Cell Biology
Genetics

Background:

Identifying transcriptional programs for stem cell identity is advanced.
Mechanisms for down-regulating these programs to enable differentiation are less understood.
Drosophila embryonic neuroblasts provide a model for temporal cell cycle progression and differentiation.

Purpose of the Study:

To elucidate the transcriptional regulation of cell cycle exit and differentiation in Drosophila neuroblasts.
To identify the genetic factors governing the switch from proliferative to non-proliferative daughter cells.

Main Methods:

Identification of key transcription factors involved in neuroblast lineage progression.
Analysis of the regulatory interactions between early and late transcription factors.
Investigation of the impact of these factors on cell-cycle gene regulation.

Main Results:

Six early transcription factors were identified that promote neuroblast and type I daughter proliferation.
Three late transcription factors were found to replace early factors, triggering the type I to type 0 daughter proliferation switch and cell cycle exit.
A genetic pathway involving mutual regulation between early and late factors and four key cell-cycle genes was established.

Conclusions:

A driver-stopper temporal program of transcription factors controls neuroblast lineage progression and cell cycle exit.
This regulatory network provides a framework for understanding stem cell differentiation timing.
The findings may have broad implications for stem cell research in other systems.