Related Experiment Video
Updated: Feb 19, 2026
09:20
An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
Published on: May 4, 2021
4.3K
Endophilin A2: A Potential Link to Adiposity and Beyond
Assim A Alfadda1,2, Reem M Sallam1,3, Rukhsana Gul1
1Obesity Research Center, College of Medicine, King Saud University, Riyadh 11461, Saudi Arabia.
Molecules and Cells
|November 9, 2017
Summary
Endophilin A2 (EA2) gene expression is higher in subcutaneous than visceral human adipose tissue. This finding suggests EA2 may play a role in obesity and metabolic disorders.
Area of Science:
- Molecular biology
- Metabolic research
- Adipose tissue biology
Background:
- Adipose tissue is crucial for inter-tissue communication.
- Understanding molecular changes in adipose tissue mass is key to addressing obesity and metabolic diseases.
- Previous research indicates a potential link between endophilins and body weight regulation.
Purpose of the Study:
- To investigate the distribution and expression patterns of endophilin A2 (EA2) in human adipose tissue.
- To explore the relationship between EA2 expression and key adipokines in different adipose depots.
- To examine EA2 expression during preadipocyte differentiation.
Main Methods:
- Analysis of human subcutaneous and visceral adipose tissue samples.
- Quantitative gene expression analysis (RT-qPCR).
- In vitro differentiation of preadipocytes.
Main Results:
- Elevated EA2 gene expression was observed in subcutaneous adipose tissue compared to visceral adipose tissue.
- EA2 expression showed negative correlations with adiponectin and chemerin in visceral fat.
- EA2 expression positively correlated with TNF-α in subcutaneous fat and decreased during adipocyte differentiation.
Conclusions:
- This study characterizes the distribution of EA2 in human adipose tissue.
- EA2 warrants further investigation for its role in the pathogenesis of obesity and associated metabolic dysfunctions.
More Related Videos
10:40Characterization of Adipocyte-Derived Extracellular Vesicle Secretion Using a CD63-GFP Reporter Mouse Model In Vivo and In Vitro
Published on: December 5, 2025
83.4K
08:34Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
Published on: June 3, 2016
15.8K
Related Concept Videos
01:24Obesity
1.4K
The Body Mass Index (BMI) is a numerical value derived from a person's weight and height, used to categorize individuals into weight ranges. It is calculated using the formula: weight in kilograms divided by height in meters squared. Obesity is a health condition characterized by excessive accumulation of adipose tissue that poses health risks, often diagnosed with a BMI ≥ 30. This excess fat storage occurs when surplus dietary calories are converted into triglycerides and stored in...
1.4K
01:02Hypodermis
7.9K
The hypodermis (the subcutaneous layer or superficial fascia) is present directly below the dermis. It connects the skin to the underlying fascia (fibrous tissue) of the bones and muscles. It is not strictly a part of the skin, although the border between the hypodermis and dermis can be difficult to distinguish. The hypodermis consists of well-vascularized, loose, areolar connective tissue and adipose tissue, which functions as a mode of fat storage and provides insulation and cushioning for...
7.9K
01:31Adrenergic Receptors: ɑ Subtype
3.0K
Adrenoceptors are classified into α and ꞵ classes based on their potencies to catecholamine agonists. α-adrenoceptors show the following order of catecholamine potency:
Adrenaline ≥ Noradrenaline >> Isoprenaline
α-adrenoceptors are further divided into α1 and α2-adrenoceptors.
α1-Adrenoceptors: These receptors are located postsynaptically on the effector organs and cause constriction of smooth muscle mediated by activation of phospholipase...
Adrenaline ≥ Noradrenaline >> Isoprenaline
α-adrenoceptors are further divided into α1 and α2-adrenoceptors.
α1-Adrenoceptors: These receptors are located postsynaptically on the effector organs and cause constriction of smooth muscle mediated by activation of phospholipase...
3.0K
01:11Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase
24
Genetic polymorphisms in drug targets have emerged as critical determinants of interindividual variability in drug response and toxicity. Pharmacogenomic investigations increasingly focus on identifying these variations to personalize and optimize therapeutic interventions. A drug target may be a receptor, enzyme, or signaling protein involved in pharmacologic responses or disease-related pathways. While early pharmacogenetic studies focused primarily on drug metabolism, current research...
24
01:25cAMP-dependent Protein Kinase Pathways
8.6K
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
8.6K
01:24Regulation of Angiogenesis and Blood Supply
3.7K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.7K